SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION IN CUCUMBER

被引:25
作者
LOU, H
KAKO, S
机构
关键词
CUCUMIS-SATIVUS; CULTIVARS; EXPLANT SOURCE; SUCROSE; ACTIVATED CHARCOAL; IN-VITRO;
D O I
10.21273/HORTSCI.29.8.906
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars was examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and 'Fushinarimidori' produced the highest number of embryos from either cotyledons or young first leaves. Cotyledon- and first-leaf-derived calluses produced more embryos than calluses from internodes. Somatic embryos were induced from 'Aonaga F1' internodes. With relatively high sucrose levels (6% and 9%) in the initiation medium, the frequency of embryogenic callus formation from 'Fushinarimidori' cotyledon explants was >90%. The highest yield of somatic embryos occurred in cultures initiated with high sucrose levels (9% or 12%), although 12% sucrose inhibited callus formation and growth. Somatic embryos germinated in a basal liquid medium supplemented with 0.5% activated charcoal, and they developed into well-shaped, healthy plantlets on semisolid medium with 1%
引用
收藏
页码:906 / 909
页数:4
相关论文
共 26 条
[21]   FLOWER FORMATION INVITRO BY HYPOCOTYL EXPLANTS OF CUCUMBER (CUCUMIS-SATIVUS L) [J].
RAJASEKARAN, K ;
MULLINS, MG ;
NAIR, Y .
ANNALS OF BOTANY, 1983, 52 (03) :417-420
[22]  
TABEI Y, 1989, B NATL RES I VEG ORN, V3, P97
[23]   INVITRO PLANT-REGENERATION IN THE GENUS CUCUMIS [J].
TRULSON, AJ ;
SHAHIN, EA .
PLANT SCIENCE, 1986, 47 (01) :35-43
[24]   FACTORS AFFECTING HAPLOID PLANT-REGENERATION FROM MAIZE ANTHER CULTURE [J].
TSAY, HS ;
MIAO, SH ;
WIDHOLM, JM .
JOURNAL OF PLANT PHYSIOLOGY, 1986, 126 (01) :33-40
[25]  
ZHOU C, 1981, PLANT SCI LETT, V20, P231
[26]   ENHANCED EMBRYOGENESIS AND PLANT-REGENERATION FROM CUCUMBER (CUCUMIS-SATIVUS L) CALLUS BY ACTIVATED-CHARCOAL IN SOLID LIQUID DOUBLE-LAYER CULTURES [J].
ZIV, M ;
GADASI, G .
PLANT SCIENCE, 1986, 47 (02) :115-122