DETERMINATION OF THE REDUCING TERMINAL SUGARS OF GLYCOSAMINOGLYCANS USING 2-AMINOPYRIDINE

被引：23

作者：

TAKAGAKI, K ^{[1
]}

NAKAMURA, T ^{[1
]}

KAWASAKI, H ^{[1
]}

KON, A ^{[1
]}

OHISHI, S ^{[1
]}

ENDO, M ^{[1
]}

机构：

[1] HIROSAKI UNIV,SCH MED,DEPT BIOCHEM,5 ZAIFU CHO,HIROSAKI,AOMORI 036,JAPAN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 03期

关键词：

2-Aminopyridine; Glycosaminoglycan; Reducing terminal;

D O I：

10.1016/0165-022X(90)90014-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorescence labeling method (Takemoto, H. et al. (1985) Anal. Biochem. 145, 245-250) has been shown to have high sensitivity for measuring the sugar composition of glycoproteins. In the present study, its applicability for analysis of the reducing terminal sugars of glycosaminoglycans was investigated. The procedure involved coupling of glycosaminoglycans with 2-aminopyridine, followed by hydrolysis and N-acetylation, and then by high-performance liquid chromatrogaphy on a reverse-phase column. The method was found to be useful for simulatenous determination of acidic, neutral and amino sugars at the reducing termini of glycosaminoglycan moieties. © 1990.

引用

页码：209 / 215

页数：7

共 14 条

[1] STRUCTURAL STUDIES ON A GLUCOSE-CONTAINING POLYSACCHARIDE OBTAINED FROM MICROCOCCUS-LYSODEIKTICUS CELL-WALLS .4. ISOLATION OF A DISACCHARIDE, 6-O-(STRONTIUM 2-ACETAMIDO-2-DEOXY-BETA-D-MANNOPYRANOSYLURONATE)-D-GLUCOSE [J].

HASE, S ;

TSUJI, Y ;

MATSUSHIMA, Y .

JOURNAL OF BIOCHEMISTRY, 1972, 72 (06) :1549-1555

[2] A HIGHLY SENSITIVE METHOD FOR ANALYSES OF SUGAR MOIETIES OF GLYCOPROTEINS BY FLUORESCENCE LABELING [J].

HASE, S ;

IKENAKA, T ;

MATSUSHIMA, Y .

JOURNAL OF BIOCHEMISTRY, 1981, 90 (02) :407-414

[3] TAGGING OF SUGARS WITH A FLUORESCENT COMPOUND, 2-AMINOPYRIDINE [J].

HASE, S ;

HARA, S ;

MATSUSHIMA, Y .

JOURNAL OF BIOCHEMISTRY, 1979, 85 (01) :217-220

[4] SELECTIVE HYDROLYSIS OF CHONDROITIN SULFATES BY HYALURONIDASE [J].

KNUDSON, W ;

GUNDLACH, MW ;

SCHMID, TM ;

CONRAD, HE .

BIOCHEMISTRY, 1984, 23 (02) :368-375

[5] IMPROVED ANALYTICAL METHOD FOR THE HEXURONIC ACIDS AT THE REDUCING TERMINALS OF GLYCOSAMINOGLYCANS [J].

MAJIMA, M ;

NAKAMURA, T ;

IGARASHI, S ;

MATSUE, H ;

ENDO, M .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1984, 9 (03) :245-249

[6] HETEROGENEITY OF REDUCING TERMINALS OF URINARY CHONDROITIN SULFATES [J].

MATSUE, H ;

ENDO, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 923 (03) :470-477

[7] CHARACTERIZATION OF A PLATELET ENDOGLYCOSIDASE DEGRADING HEPARIN-LIKE POLYSACCHARIDES [J].

OLDBERG, A ;

HELDIN, CH ;

WASTESON, A ;

BUSCH, C ;

HOOK, M .

BIOCHEMISTRY, 1980, 19 (25) :5755-5762

[8]

Roden L., 1980, BIOCH GLYCOPROTEINS, P267

[9]

TAKAGAKI K, 1990, J BIOL CHEM, V265, P854

[10]

TAKAGAKI K, 1988, J BIOL CHEM, V263, P7000

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