N-6 POLYUNSATURATED FATTY-ACIDS INCREASE THE NEURITE LENGTH OF PC12 CELLS AND EMBRYONIC CHICK MOTONEURONS

被引：21

作者：

DEHAUT, F ^{[1
]}

BERTRAND, I ^{[1
]}

MILTAUD, T ^{[1
]}

POUPLARDBARTHELAIX, A ^{[1
]}

MAINGAULT, M ^{[1
]}

机构：

[1] CHRU ANGERS,BIOL CELLULAIRE LAB,F-49033 ANGERS,FRANCE

来源：

NEUROSCIENCE LETTERS | 1993年 / 161卷 / 02期

关键词：

POLYUNSATURATED FATTY ACID; PC12; CELL; EMBRYONIC CHICK MOTONEURON; NEURITE OUTGROWTH;

D O I：

10.1016/0304-3940(93)90277-R

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have tested the action of three n-6 polyunsaturated fatty acids, either free or in the form of ethyl esters, on the neurite outgrowth in two neuronal models: a rat pheochromocytoma cell line (PC12) and embryonic chick motoneurons, after 7 days in culture. An inverted microscope coupled with the 'VIDS 4' software was used for measuring the neurite length. Free fatty acids were found to be cytotoxic at 10(-3) M and the maximal increase of the neurite length was obtained at 10(-5) M. In contrast, fatty acids in the form of ethylesters were not cytotoxic and at 10(-3) M induced the maximal increase in the neurite length. This increase (1.2 to 2 fold) significantly differed from the control and was dose-dependent. These results were discussed in relation to the action of fatty acids on enzyme activation and membrane fluidity.

引用

页码：133 / 136

页数：4

共 19 条

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[2]

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