DETERMINATION OF SERUM CREATININE BY ISOTOPE-DILUTION METHOD USING DISCHARGE-ASSISTED THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：12

作者：

TAKATSU, A

NISHI, S

机构：

[1] National Institute of Materials and Chemical Research, Tsukuba, Ibaraki

来源：

BIOLOGICAL MASS SPECTROMETRY | 1993年 / 22卷 / 11期

关键词：

D O I：

10.1002/bms.1200221104

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A discharge-assisted thermospray (plasmaspray) liquid chromatography/mass spectrometric method for the determination of serum creatinine is described. The method incorporates stable isotope dilution using (D3)creatinine as an internal standard. Separation is performed in reversed-phase high-performance liquid chromatography using 0.01 M aqueous ammonium acetate as a flow solvent. Effluents are directly introduced to the mass spectrometer and [MH]+ ions are monitored during LC/MS using the selected ion monitoring method. Satisfactory agreement between the analytical result and the certified value of the serum sample of standard reference material and relative standard deviation ranging from 0.6% to 1.2% was obtained.

引用

页码：643 / 646

页数：4

共 13 条

[1]

BJORKHEM I, 1977, CLIN CHEM, V23, P1114

[2] EXPERIMENTS ON A CYANOGUANADINO COMPOUND USING DISCHARGE-ASSISTED THERMOSPRAY IONIZATION [J].