INSULIN-LIKE GROWTH FACTOR-I (IGF-I) STIMULATES PROTEIN-SYNTHESIS AND COLLAGEN GENE-EXPRESSION IN MONOLAYER AND LATTICE CULTURES OF FIBROBLASTS

被引:93
作者
GILLERY, P
LEPERRE, A
MAQUART, FX
BOREL, JP
机构
[1] Laboratory of Biochemistry, Cnrs Ura 610, Faculty of Medicine, University of Reims Champagne-Ardenne, Reims
关键词
D O I
10.1002/jcp.1041520221
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fibroblasts cultivated in three-dimensional tissue-like matrices are characterized by a slowed metabolism and a decrease of protein synthesis, unless they are submitted to physical tensions. We checked the effects of insulin like growth factor-I (IGF-I), known as a potent stimulator of mitogenesis and protein synthesis for many cell types, in various models of cultures: confluent monolayers, collagen lattices, non-retracting or retracting fibrin lattices. IGF-I (1-100 ng.ml-1) had no effect on cell divisions in lattice cultures. It was able to stimulate collagen lattice retraction when the medium was supplemented with low concentrations of serum. IGF-I at 10 or 100 ng.ml-1 stimulated collagen and non-collagen syntheses in all culture systems, but stimulation of collagen synthesis only began at the highest concentration (100 ng.ml-1) in retracted lattices. Northern blot and dot-blot analyses of mRNAs extracted from monolayer cultures of fibroblasts showed that IGF-I stimulated pro alpha-1(I) collagen synthesis at the pretranslational level. Cycloheximide (7.5-mu-g.ml-1) completely inhibited pro alpha-1(I) collagen gene expression induced by IGF-I. These results show that IGF-I is a potent stimulus for protein synthesis and collagen gene expression in monolayers and tridimensional cultures of fibroblasts, but that it exerts no mitogenic activity in tridimensional lattices. Synergistic associations of IGF-I with other growth factors will have to be found in order to reverse the quiescent status of fibroblasts in lattices.
引用
收藏
页码:389 / 396
页数:8
相关论文
共 29 条
[1]   PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].
BELL, E ;
IVARSSON, B ;
MERRILL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) :1274-1278
[2]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3]   PLATELET ISOFORMS OF PLATELET-DERIVED GROWTH-FACTOR STIMULATE FIBROBLASTS TO CONTRACT COLLAGEN MATRICES [J].
CLARK, RAF ;
FOLKVORD, JM ;
HART, CE ;
MURRAY, MJ ;
MCPHERSON, JM .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (03) :1036-1040
[4]   RESPONSE TO EPIDERMAL GROWTH-FACTOR OF SKIN FIBROBLASTS FROM DONORS OF VARYING AGE IS MODULATED BY THE EXTRACELLULAR-MATRIX [J].
COLIGE, A ;
NUSGENS, B ;
LAPIERE, CM .
JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 145 (03) :450-457
[5]   EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON FIBROBLASTS IN 3-DIMENSIONAL LATTICE CULTURES [J].
COUSTRY, F ;
GILLERY, P ;
MAQUART, FX ;
BOREL, JP .
FEBS LETTERS, 1990, 262 (02) :339-341
[6]   ACTIVITIES OF HUMAN ACIDIC FIBROBLAST GROWTH-FACTOR IN AN INVITRO DERMAL EQUIVALENT MODEL [J].
DUBERTRET, L ;
BRUNNERFERBER, F ;
MISITI, J ;
THOMAS, KA ;
DUBERTRET, ML .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (05) :793-798
[7]   A GENERAL, FAST, AND SENSITIVE MICROMETHOD FOR DNA DETERMINATION - APPLICATION TO RAT AND MOUSE-LIVER, RAT HEPATOMA, HUMAN-LEUKOCYTES, CHICKEN FIBROBLASTS, AND YEAST-CELLS [J].
FISZERSZAFARZ, B ;
SZAFARZ, D ;
DEMURILLO, AG .
ANALYTICAL BIOCHEMISTRY, 1981, 110 (01) :165-170
[8]  
FROESCH ER, 1985, ANNU REV PHYSIOL, V47, P443
[9]   ISOLATION AND CHARACTERIZATION OF PEPSIN-TREATED TYPE-3 COLLAGEN FROM CALF SKIN [J].
FUJII, T ;
KUHN, K .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1975, 356 (11) :1793-1801
[10]   CULTURES OF FIBROBLASTS IN FIBRIN LATTICES - MODELS FOR THE STUDY OF METABOLIC-ACTIVITIES OF THE CELLS IN PHYSIOLOGICAL CONDITIONS [J].
GILLERY, P ;
BELLON, G ;
COUSTRY, F ;
BOREL, JP .
JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 140 (03) :483-490