DETERMINATION OF NON-VOLATILE N-NITROSO COMPOUNDS IN BIOLOGICAL-FLUIDS BY LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN PHOTOHYDROLYSIS DETECTION

被引：34

作者：

SHUKER, DEG ^{[1
]}

TANNENBAUM, SR ^{[1
]}

机构：

[1] MIT,DEPT NUTR & FOOD SCI,CAMBRIDGE,MA 02139

来源：

ANALYTICAL CHEMISTRY | 1983年 / 55卷 / 13期

关键词：

D O I：

10.1021/ac00263a034

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A modified photohydrolysis detector (PHD) has been developed for use with reversed-phase HPLC. With the HPLC-PHD system nonvolatile N-nitroso compounds (particularly N-nitrosamides) were selectively determined following chromatographic separation. Light from a high-intensity discharge lamp photolyzes N-nitroso compounds in aqueous solution to give nitrite ion, which was determined colorimetrically with Griess reagent in a postcolumn reactor. Several N-nitroso compounds gave a positive response (detection limit, ng/injection): N-methyl-N prime -nitro-N-nitrosoguanidine (8), N-methyl-N-nitrosourea (20), N-methyl-N-nitrosoacetamide (8), N-nitrosotaurocholic acid (100), N-nitrosoglycocholic acid (60), N-nitrosocimetidine (6), N-nitroso-L-proline (20). Methods were developed for the analysis of N-nitrosoglycocholic acid in gastric juice and N-nitrosocimetidine in gastric juice and urine.

引用

页码：2152 / 2155

页数：4

共 16 条

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