HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PYRROLOQUINOLINE QUINONE AS ACETONE ADDUCT

被引：7

作者：

KANO, K

UNO, B

KAWASAKI, C

HORIKI, K

KAWAI, S

机构：

[1] Gifu Pharmaceutical University, Mitahora-higashi

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷 / 05期

关键词：

PYRROLOQUINOLINE QUINONE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ACETONE ADDUCT; ULTRAVIOLET DETECTION;

D O I：

10.2116/analsci.7.737

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high performance liquid chromatographic method for the determination of pyrroloquinoline quinone (PQQ) is described. PQQ reacts readily with acetone in a weakly alkaline medium, giving a stable adduct, 5-acetonyl PQQ. The adduct was separated on a Develosil C18-5 column using a mixture of methanol and 0.06 M phosphoric acid, and was detected at 254 nm. The response was linear over the range (1- 20) x 10(-6) M of PQQ and the detection limit was 2 pmol for a 20-mu-l injection volume.

引用

页码：737 / 739

页数：3

共 12 条

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