RECOMBINANT 2-DEOXYRIBOSE-5-PHOSPHATE ALDOLASE IN ORGANIC-SYNTHESIS - USE OF SEQUENTIAL 2-SUBSTRATE AND 3-SUBSTRATE ALDOL REACTIONS

被引：83

作者：

WONG, CH

GARCIAJUNCEDA, E

CHEN, LR

BLANCO, O

GIJSEN, HJM

STEENSMA, DH

机构：

[1] Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1995年 / 117卷 / 12期

关键词：

D O I：

10.1021/ja00117a003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new procedure has been developed for the large scale preparation of recombinant 2-deoxyribose-5-phosphate aldolase (DERA, EC 4.1.2.4) from E. coli strain DH5 alpha (ATCC 86963). The enzyme was purified to homogeneity with an overall yield of 83% and in sufficient quantity to grow crystals suitable for X-ray diffraction studies. Using the sequential two- or three-substrate aldol reaction, DERA was applied to the synthesis of a variety of sugar analogs including deoxyriboses, 2-deoxyfucose analogs, dideoxyhexoses, trideoxyhexoses, deoxythiosugars, and C-13-substituted 2-deoxyribose-5-phosphate.

引用

页码：3333 / 3339

页数：7

共 27 条

[21] ENZYMES IN ORGANIC-SYNTHESIS - SYNTHESIS OF HIGHLY ENANTIOMERICALLY PURE 1,2-EPOXY ALDEHYDES, EPOXY ALCOHOLS, THIIRANE, AZIRIDINE, AND GLYCERALDEHYDE-3-PHOSPHATE [J].