COMPARATIVE NUCLEAR-MAGNETIC-RESONANCE STUDIES OF DIFFUSIONAL WATER PERMEABILITY OF RED-BLOOD-CELLS FROM SHEEP AND COW

被引:24
作者
BENGA, G
BORZA, T
POPESCU, O
PORUTIU, D
MATEI, H
机构
[1] Department of Cell and Molecular Biology, University of Medicine and Pharmacy-Cluj-Napoca, 3400 Cluj-Napoca
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 104卷 / 03期
基金
英国惠康基金;
关键词
D O I
10.1016/0305-0491(93)90286-E
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. The diffusional water permeability (p) of sheep and cow red blood cell (RBC) membrane has been monitored by a doping nuclear magnetic resonance (NMR) technique on control cells and following inhibition with p-chloromercuribenzene sulfonate (PCMBS). 2. There were no significant differences in the water permeability of sheep and cow RBCs, the values of p being around 3 x 10(-3) cm/sec at 20-degrees-C and 5 x 10(-3) cm/sec at 37-degrees-C. 3. Systematic studies of the effects of PCMBS on water diffusion indicated that in both species the maximal inhibition is reached in 60-90 min at 37-degrees-C with 1 mM PCMBS. 4. The degree of inhibition increased as the temperature of measurement decreased, regardless of PCMBS concentration and incubation time. 5. The values of maximal inhibition ranged from 60-70% at 20-degrees-C to 50-60% at 37-degrees-C in the case of sheep RBCs, and from 45-55% at 20-degrees-C to 40-50% at 37-degrees-C in the case of cow RBCs. 6. The basal permeability to water of sheep RBCs was estimated as 1.0 x 10(-3) cm/sec at 20-degrees-C and 2.2 x 10(-3) cm/sec at 37-degrees-C, and that of cow RBCs as 1.6 x 10(-3) cm/sec at 20-degrees-C and 2.7 x 10(-3) cm/sec at 37-degrees-C. 7. In both species the activation energy of water diffusion was around 23 kJ/mol in control cells and reached values of around 30 kJ/mol after incubation with PCMBS in conditions of maximal inhibition of water diffusion. 8. The membrane polypeptide electrophoretic patterns of sheep and cow RBCs have been compared with their human counterparts. 9. The proteins migrating in bands 1 and 2 (corresponding to spectrins) and in band 6 (glyceraldehyde-3-phosphate dehydrogenase) were less abundant in sheep and cow RBCs compared to human RBCs. 10. There were also differences between sheep and cow RBC membrane proteins. 11. Proteins in bands 2.1-2.3 (ankyrins) and band 3 were decreased in cow RBC membrane. 12. These changes in membrane proteins did not seem to correlate with significant differences in the diffusional permeability to water of RBCs in these species.
引用
收藏
页码:589 / 594
页数:6
相关论文
共 37 条
[1]   ON MEASURING THE DIFFUSIONAL WATER PERMEABILITY OF HUMAN RED-BLOOD-CELLS AND GHOSTS BY NUCLEAR-MAGNETIC-RESONANCE [J].
BENGA, G ;
POP, VI ;
POPESCU, O ;
BORZA, V .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1990, 21 (02) :87-102
[2]  
BENGA G, 1989, INT REV CYTOL, V114, P273
[3]  
BENGA G, 1990, REV ROUM BIOCHIM, V27, P189
[4]   WATER EXCHANGE THROUGH ERYTHROCYTE-MEMBRANES - NUCLEAR MAGNETIC-RESONANCE STUDIES ON THE EFFECTS OF INHIBITORS AND OF CHEMICAL MODIFICATION OF HUMAN MEMBRANES [J].
BENGA, G ;
POP, VI ;
POPESCU, O ;
IONESCU, M ;
MIHELE, V .
JOURNAL OF MEMBRANE BIOLOGY, 1983, 76 (02) :129-137
[5]   WATER EXCHANGE THROUGH ERYTHROCYTE-MEMBRANES - BIOCHEMICAL AND NUCLEAR MAGNETIC-RESONANCE STUDIES RE-EVALUATING THE EFFECTS OF SULFHYDRYL-REAGENTS AND OF PROTEOLYTIC-ENZYMES ON HUMAN MEMBRANES [J].
BENGA, G ;
POPESCU, O ;
BORZA, V ;
POP, VI ;
HODARNAU, A .
JOURNAL OF MEMBRANE BIOLOGY, 1989, 108 (02) :105-113
[6]   WATER TRANSPORT IN HUMAN RED-CELLS - EFFECTS OF NONINHIBITORY SULFHYDRYL-REAGENTS [J].
BENGA, G ;
POPESCU, O ;
BORZA, V ;
HODARNAU, A ;
POP, VI ;
WRIGGLESWORTH, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1061 (02) :309-312
[7]   WATER TRANSPORT IN RED BLOOD-CELL MEMBRANES [J].
BENGA, G .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1988, 51 (03) :193-245
[8]   THE BASAL PERMEABILITY TO WATER OF HUMAN RED-BLOOD-CELLS EVALUATED BY A NUCLEAR-MAGNETIC-RESONANCE TECHNIQUE [J].
BENGA, G ;
POP, VI ;
POPESCU, O ;
BORZA, V .
BIOSCIENCE REPORTS, 1990, 10 (01) :31-36
[9]   EFFECTS OF TEMPERATURE ON WATER DIFFUSION IN HUMAN-ERYTHROCYTES AND GHOSTS - NUCLEAR-MAGNETIC-RESONANCE STUDIES [J].
BENGA, G ;
POP, VI ;
POPESCU, O ;
HODARNAU, A ;
BORZA, V ;
PRESECAN, E .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 905 (02) :339-348
[10]   WATER DIFFUSION PERMEABILITY OF ERYTHROCYTES USING AN NMR TECHNIQUE [J].
CONLON, T ;
OUTHRED, R .
BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 288 (02) :354-&