EFFECT OF PROTEIN-KINASE-C INHIBITORS ON THE ANTIVIRAL ACTIVITY OF HUMAN ALPHA-INTERFERON IN HERPES-SIMPLEX VIRUS-INFECTED HUMAN NEUROBLASTOMA-CELLS

被引：4

作者：

CHATTERJEE, S ^{[1
]}

BURNS, P ^{[1
]}

KOGA, J ^{[1
]}

机构：

[1] JCR PHARMACEUT,KOBE 65122,JAPAN

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 02期

关键词：

D O I：

10.1128/JVI.69.2.1315-1318.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pretreatment of human neuroblastoma cells with an inhibitor of protein kinase C (PKC), staurosporine or H-7, prior to the addition of human alpha interferon (HuIFN-alpha), recombinant HuIFN-alpha, or recombinant HuIFN-beta blocked the inhibitory effect of these IFNs on the release of infectious herpes simplex virus type 1 from treated cells. In addition, staurosporine blocked the inhibitory effect of HuIFNs on the expressions of herpes simplex type 1 glycoproteins B, C, and D in treated neuroblastoma cells. Furthermore, addition of HuIFNs resulted in an increased expression of PKC in treated neuroblastoma cells. These results suggest that inhibitors of PKC block the expression of HuIFN-induced genes in treated human neuroblastoma cells. Thus, the activation of PKC is an important step in the HuIFN-treated cells of neuronal origin.

引用

页码：1315 / 1318

页数：4

共 26 条

[21] TRANSMEMBRANE SIGNALING BY INTERFERON-ALPHA INVOLVES DIACYLGLYCEROL PRODUCTION AND ACTIVATION OF THE EPSILON-ISOFORM OF PROTEIN-KINASE-C IN DAUDI CELLS [J].