SEPARATION AND CHARACTERIZATION OF PYRIMIDINE DEOXYRIBONUCLEOSIDE 2'-HYDROXYLASE AND THYMINE 7-HYDROXYLASE FROM ASPERGILLUS-NIDULANS

被引：5

作者：

SHAFFER, PM ^{[1
]}

CAIRNS, JR ^{[1
]}

DORMAN, DC ^{[1
]}

LOTT, AM ^{[1
]}

机构：

[1] UNIV SAN DIEGO, DEPT PHYS SCI, SAN DIEGO, CA 92110 USA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1984年 / 16卷 / 04期

关键词：

D O I：

10.1016/0020-711X(84)90143-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partially purified preparations from A. nidulans catalyze 2 .alpha.-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2''-hydroxylase (EC 1.14.11.3) and the thymine 7-hydroxylase (EC 1.14.11.6) reactions. These reactions showed an absolute requirement for .alpha.-ketoglutarate and O2 and were stimulated by Fe(II), ascorbate and catalase. Both reactions demonstrated a stoichiometry such that for each mole of substrate (deoxyribonucleoside or pyrimidine) hydroxylated 1 mol of CO2 was produced from .alpha.-ketoglutarate. These 2 activities were separated using DEAE-Sephacel chromatography.

引用

页码：429 / 434

页数：6

共 18 条

[1]

Abbott MT, 1974, MOL MECHANISMS OXYGE, P167

[2] NITROGEN METABOLITE REPRESSION IN ASPERGILLUS-NIDULANS [J].

ARST, HN ;

COVE, DJ .

MOLECULAR & GENERAL GENETICS, 1973, 126 (02) :111-141

[3] NITROGEN METABOLITE REPRESSION OF FLUOROPYRIMIDINE RESISTANCE AND PYRIMIDINE UPTAKE IN NEUROSPORA-CRASSA [J].

BUXTON, FP ;

RADFORD, A .

MOLECULAR & GENERAL GENETICS, 1982, 186 (02) :259-262

[4] SYNTHESIS OF 5-SUBSTITUTED PYRIMIDINES VIA FORMALDEHYDE ADDITION [J].

CLINE, RE ;

FINK, RM ;

FINK, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1959, 81 (10) :2521-2527

[5] INDUCTION AND REPRESSION OF NITRATE REDUCTASE IN FUNGUS ASPERGILLUS NIDULANS [J].

COVE, DJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 113 (01) :51-&

[6] PAPER CHROMATOGRAPHY OF SEVERAL CLASSES OF COMPOUNDS - CORRELATED RF VALUES IN A VARIETY OF SOLVENT SYSTEMS [J].

FINK, K ;

FINK, RM ;

CLINE, RE .

ANALYTICAL CHEMISTRY, 1963, 35 (03) :389-&

[7] REGULATION OF THYMIDINE METABOLISM IN NEUROSPORA-CRASSA [J].

GRISWOLD, WR ;

MADRID, VO ;

SHAFFER, PM ;

TAPPEN, DC ;

PUGH, CSG ;

ABBOTT, MT .

JOURNAL OF BACTERIOLOGY, 1976, 125 (03) :1040-1047

[8]

Hayaishi O., 1975, ENZYMES, V12, P119

[9] CATALYSIS OF 3 SEQUENTIAL DIOXYGENASE REACTIONS BY THYMINE 7-HYDROXYLASE [J].

LIU, CK ;

HSU, CA ;

ABBOTT, MT .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 159 (01) :180-187

[10] STUDIES PERTAINING TO PURIFICATION AND PROPERTIES OF THYMINE 7-HYDROXYLASE [J].

MCCROSKEY, RP ;

GRISWOLD, WR ;

SOKOLOFF, RL ;

SEVIER, ED ;

LIN, S ;

LIU, CK ;

SHAFFER, PM ;

PALMATIER, RD ;

PARKER, TS ;

ABBOTT, MT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 227 (02) :264-+

← 1 2 →