SEPARATION AND CHARACTERIZATION OF PYRIMIDINE DEOXYRIBONUCLEOSIDE 2'-HYDROXYLASE AND THYMINE 7-HYDROXYLASE FROM ASPERGILLUS-NIDULANS

被引：5

作者：

SHAFFER, PM ^{[1
]}

CAIRNS, JR ^{[1
]}

DORMAN, DC ^{[1
]}

LOTT, AM ^{[1
]}

机构：

[1] UNIV SAN DIEGO, DEPT PHYS SCI, SAN DIEGO, CA 92110 USA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1984年 / 16卷 / 04期

关键词：

D O I：

10.1016/0020-711X(84)90143-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partially purified preparations from A. nidulans catalyze 2 .alpha.-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2''-hydroxylase (EC 1.14.11.3) and the thymine 7-hydroxylase (EC 1.14.11.6) reactions. These reactions showed an absolute requirement for .alpha.-ketoglutarate and O2 and were stimulated by Fe(II), ascorbate and catalase. Both reactions demonstrated a stoichiometry such that for each mole of substrate (deoxyribonucleoside or pyrimidine) hydroxylated 1 mol of CO2 was produced from .alpha.-ketoglutarate. These 2 activities were separated using DEAE-Sephacel chromatography.

引用

页码：429 / 434

页数：6

共 18 条

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