ISOLATION AND COMPARATIVE MAPPING OF A HUMAN-CHROMOSOME 20-SPECIFIC ALPHA-SATELLITE DNA CLONE

被引：24

作者：

BALDINI, A

ARCHIDIACONO, N

CARBONE, R

BOLINO, A

SHRIDHAR, V

MILLER, OJ

MILLER, DA

WARD, DC

ROCCHI, M

机构：

[1] UNIV BARI,IST GENET,VIA AMENDOLA 165-A,I-70126 BARI,ITALY

[2] WAYNE STATE UNIV,SCH MED,DEPT MOLEC BIOL & GENET,DETROIT,MI 48201

[3] YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510

[4] IST GIANNINA GASLINI,I-16148 GENOA,ITALY

[5] WAYNE STATE UNIV,SCH MED,CTR MOLEC BIOL,DETROIT,MI 48201

[6] CNR,INST MOLEC GENET,PORTO CONTE RES & TRAINING LABS,SASSARI,ITALY

来源：

CYTOGENETICS AND CELL GENETICS | 1992年 / 59卷 / 01期

关键词：

D O I：

10.1159/000133188

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.

引用

页码：12 / 16

页数：5

共 44 条

[41] DETECTION OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AT THE CENTROMERES OF HUMAN-CHROMOSOMES BY USING CHROMOSOME-SPECIFIC ALPHA-SATELLITE DNA PROBES - IMPLICATIONS FOR DEVELOPMENT OF CENTROMERE-BASED GENETIC-LINKAGE MAPS [J].