EFFECTS OF SUBUNIT TYPES OF THE CLONED GABA-A RECEPTOR ON THE RESPONSE TO A NEUROSTEROID

被引：137

作者：

SHINGAI, R

SUTHERLAND, ML

BARNARD, EA

机构：

[1] MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, CB2 2QH, Hills Road

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1991年 / 206卷 / 01期

关键词：

NEUROSTEROID; GABA-A RECEPTORS; CLONED SUBTYPES; ELECTROPHYSIOLOGY;

D O I：

10.1016/0922-4106(91)90149-C

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Combinations of cloned GABA(A) receptor subtypes, having the subunit combinations alpha-i + beta-1 or alpha-i + beta-1 + gamma-2 (i = 1, 2, 3), were expressed in Xenopus oocytes. The endogenous steroid 3-alpha-hydroxy-5-alpha-pregnan-20-one potentiates GABA currents induced therein by GABA. This potentiation was greater in the alpha-1 + beta and alpha-3 + beta-1 than in the alpha-2 + beta-1 combinations. The presence of the gamma-2-subunit increased the steroid potency in alpha-1 + beta-1 and alpha-2 + beta-1, but the combination alpha-3 + beta-1 + gamma-2 became much less steroid-sensitive. It is concluded that the steroid modification of the GABA(A) receptor is strongly influenced by the alpha- and the gamma-2-subunit types.

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页码：77 / 80

页数：4

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