PHOTOSENSITIVITY OF A PROTOPORPHYRIN-ACCUMULATING, LIGHT-SENSITIVE MUTANT (VISA) OF ESCHERICHIA-COLI K-12

被引：69

作者：

NAKAHIGASHI, K ^{[1
]}

NISHIMURA, K ^{[1
]}

MIYAMOTO, K ^{[1
]}

INOKUCHI, H ^{[1
]}

机构：

[1] KYOTO UNIV, FAC SCI, DEPT BIOPHYS, SAKYO KU, KYOTO 606, JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期

关键词：

HEME; ACTIVE OXYGEN; PROTOPORPHYRIN-IX; FERROCHELATASE;

D O I：

10.1073/pnas.88.23.10520

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mutations in the visA gene of Escherichia coli cause the mutant bacteria to die upon illumination with visible light. We confirmed genetically that the visA gene is a structural gene for ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1). Since other mutations in the genes involved in the biosynthesis of heme can cure the photosensitivity, the light-induced cell death appears to be brought about by the accumulation of protoporphyrin IX, one of the substrates of ferrochelatase. When cells are illuminated with visible light, protoporphyrin IX seems to produce an active species of oxygen (probably 1O2) that is harmful to the cells. This defect is the same as that associated with the human disease protoporphyria.

引用

页码：10520 / 10524

页数：5

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