AMPLIFICATION OF SALMONELLA CHROMOSOMAL DNA USING THE POLYMERASE CHAIN-REACTION

被引：26

作者：

NGUYEN, AV ^{[1
]}

KHAN, MI ^{[1
]}

LU, ZQ ^{[1
]}

机构：

[1] UNIV CONNECTICUT,COLL AGR & NAT RESOURCES,DEPT PATHOBIOL,STORRS,CT 06269

来源：

AVIAN DISEASES | 1994年 / 38卷 / 01期

关键词：

D O I：

10.2307/1591844

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A Salmonella-specific polymerase chain reaction (PCR) was developed and standardized. The origin of the primers was a recombinant clone (C7) that contained Salmonella-specific HindIII fragment DNA of 2.1-kilobase pairs. Based on the sequence data of Salmonella enteritidis recombinant clone C7, two primers designated NK1 (21 nucleotides) and NK2 (24 nucleotides) were synthesized for use in the PCR. A Salmonella-specific 2.0-kilobase pair DNA product was amplified by the primers from 23 species of Salmonella, but not from 19 enteric and non-enteric bacteria. As little as 330 fg of Salmonella DNA was detected using either ethidium bromide/ultraviolet exposure of gels or Southern blot hybridization with a C7 clone.

引用

页码：119 / 126

页数：8

共 25 条

[11] RAPID AND SENSITIVE DETECTION OF SALMONELLA (O-6,7) BY IMMUNOMAGNETIC MONOCLONAL ANTIBODY-BASED ASSAYS [J].

LUK, JMC ;

LINDBERG, AA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 137 (01) :1-8

[12] SEMISOLID MEDIA FOR ISOLATION OF SALMONELLA SPP FROM COASTAL WATERS [J].

PERALES, I ;

AUDICANA, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (11) :3032-3033

[13] AMPLIFICATION OF AN INVA GENE SEQUENCE OF SALMONELLA-TYPHIMURIUM BY POLYMERASE CHAIN-REACTION AS A SPECIFIC METHOD OF DETECTION OF SALMONELLA [J].

RAHN, K ;

DEGRANDIS, SA ;

CLARKE, RC ;

MCEWEN, SA ;

GALAN, JE ;

GINOCCHIO, C ;

CURTISS, R ;

GYLES, CL .

MOLECULAR AND CELLULAR PROBES, 1992, 6 (04) :271-279

[14]

Rubin R.H., 1977, SALMONELLOSIS MICROB

[15] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

[16]

Sambrook J., 1989, MOL CLONING

[17] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[18] DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS [J].

SOUTHERN, EM .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) :503-+

[19] THE EMERGENCE OF GRADE-A EGGS AS A MAJOR SOURCE OF SALMONELLA-ENTERITIDIS INFECTIONS - NEW IMPLICATIONS FOR THE CONTROL OF SALMONELLOSIS [J].

STLOUIS, ME ;

MORSE, DL ;

POTTER, ME ;

DEMELFI, TM ;

GUZEWICH, JJ ;

TAUXE, RV ;

BLAKE, PA .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1988, 259 (14) :2103-2107

[20]

SVENUNGSSON B, 1978, ACTA PATH MICRO IM B, V86, P283

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