THE CARBONIC-ANHYDRASE DOMAIN OF RECEPTOR TYROSINE PHOSPHATASE-BETA IS A FUNCTIONAL LIGAND FOR THE AXONAL CELL RECOGNITION MOLECULE CONTACTIN

被引：350

作者：

PELES, E ^{[1
]}

NATIV, M ^{[1
]}

CAMPBELL, PL ^{[1
]}

SAKURAI, T ^{[1
]}

MARTINEZ, R ^{[1
]}

LEV, S ^{[1
]}

CLARY, DO ^{[1
]}

SCHILLING, J ^{[1
]}

BARNEA, G ^{[1
]}

PLOWMAN, GD ^{[1
]}

GRUMET, M ^{[1
]}

SCHLESSINGER, J ^{[1
]}

机构：

[1] NYU,MED CTR,DEPT PHARMACOL,NEW YORK,NY 10016

来源：

CELL | 1995年 / 82卷 / 02期

关键词：

D O I：

10.1016/0092-8674(95)90312-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Receptor-type protein tyrosine phosphatase beta (RPTP beta) is expressed in the developing nervous system and contains a carbonic anhydrase (CAH) domain as well as a fibronectin type III repeat in its extracellular domain. Fusion proteins containing these domains were used to search for ligands of RPTP beta. The CAH domain bound specifically to a 140 kDa protein expressed on the surface of neuronal cells. Expression cloning in COS7 cells revealed that this protein is contactin, a GPI membrane-anchored neuronal cell recognition molecule. The CAH domain of RPTP beta induced cell adhesion and neurite growth of primary tectal neurons, and differentiation of neuroblastoma cells. These responses were blocked by antibodies against contactin, demonstrating that contactin is a neuronal receptor for RPTP beta. These experiments show that an individual domain of RPTP beta acts as a functional ligand for the neuronal receptor contactin. The Interaction between contactin and RPTP beta may generate unidirectional or bidirectional signals during neural development.

引用

页码：251 / 260

页数：10

共 57 条

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