Effects of rebamipide on gastric cell damage by Helicobacter pylori-stimulated human neutrophils

被引:28
作者
Han, BG
Kim, HS
Rhee, KH
Han, HS
Chung, MH
机构
[1] SEOUL NATL UNIV,COLL MED,DEPT PHARMACOL,CHONGNO GU,SEOUL 110799,SOUTH KOREA
[2] GYEONGSANG NATL UNIV,COLL MED,DEPT MICROBIOL,KYUNGSANG NAM DO,SOUTH KOREA
[3] EWHA WOMANS UNIV,COLL MED,DEPT SURG,YANGCHUN GU,SEOUL 158056,SOUTH KOREA
关键词
gastric ulcer; oxygen radicals; H-pylori; neutrophil; rebamipide;
D O I
10.1016/S1043-6618(05)80023-4
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Helicobacter pylori stimulated human neutrophils to produce oxygen radicals as evidenced by the production of chemiluminescence in the presence of luminol. The capacity of H. pylori to produce oxygen radicals from neutrophils was much higher than that of Escherichia coli and Staphylococcus aureus and is almost as strong as that of PMA. Rebamipide (2-(4-chlorobenzoylamino)-3-[2-(1H)-quinolinon-4-yl] propionic acid) suppressed the chemiluminescence produced by H. pylori-stimulated neutrophils and also suppressed the chemiluminescence produced by a cell-free xanthine/xanthine oxidase reaction with luminol. Thus, it is indicated that this drug has the action of scavenging oxygen radicals. Gastric mucosal cells labelled with a fluorescent dye were damaged by the incubation of the cells with neutrophils and H. pylori, and this damage was protected by rebamipide. The protection of cell damage was ascertained as a decrease in the release of fluorescent dye into the incubation medium and a reduction in the distortion of cell geometry. The data suggest that H. pylori induce human neutrophils to produce oxygen radicals which are responsible for gastric mucosal cell damage and that rebamipide removes the oxygen radicals produced from H. pylori-activated neutrophils and thus reduces the gastric mucosal cell damage. These effects may account for the ulcerogenesis action of H. pylori and for part of the mechanism of the anti-ulcer action of rebamipide. (C) 1995 The Italian Pharmacological Society
引用
收藏
页码:201 / 207
页数:7
相关论文
共 27 条
[1]   SEQUENCE OF GASTRIC-MUCOSAL INJURY FOLLOWING ISCHEMIA AND REPERFUSION - ROLE OF REACTIVE OXYGEN METABOLITES [J].
ANDREWS, FJ ;
MALCONTENTI, C ;
OBRIEN, PE .
DIGESTIVE DISEASES AND SCIENCES, 1992, 37 (09) :1356-1361
[2]  
Baik SC, 1990, J KOREAN SOC MICROBI, V25, P455
[3]   HELICOBACTER-PYLORI SECRETES A CHEMOTACTIC FACTOR FOR MONOCYTES AND NEUTROPHILS [J].
CRAIG, PM ;
TERRITO, MC ;
KARNES, WE ;
WALSH, JH .
GUT, 1992, 33 (08) :1020-1023
[4]  
DECHATELET LR, 1982, J IMMUNOL, V129, P1589
[5]   CAMPYLOBACTER-PYLORI AND PEPTIC-ULCER DISEASE [J].
GRAHAM, DY .
GASTROENTEROLOGY, 1989, 96 (02) :615-625
[6]   A COMPARATIVE-STUDY OF NEUTROPHIL PURIFICATION AND FUNCTION [J].
GRISHAM, MB ;
ENGERSON, TD ;
MCCORD, JM ;
JONES, HP .
JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 82 (02) :315-320
[7]   OXIDANT-SCAVENGING ACTIVITIES OF AMPICILLIN AND SULBACTAM AND THEIR EFFECTS ON NEUTROPHIL FUNCTIONS [J].
GUNTHER, MR ;
MAO, JH ;
COHEN, MS .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (05) :950-956
[8]   PROTECTIVE EFFECT OF REBAMIPIDE (OPC-12759) ON THE GASTRIC-MUCOSA IN RATS AND HUMANS [J].
KAWANO, S ;
SATO, N ;
KAMADA, T ;
YAMASAKI, K ;
IMAIZUMI, T ;
KOMEMUSHI, S .
FOLIA PHARMACOLOGICA JAPONICA, 1991, 97 (06) :371-380
[9]   MEASUREMENT OF CYTO-TOXICITY BY TARGET-CELL RELEASE AND RETENTION OF THE FLUORESCENT DYE BIS-CARBOXYETHYL-CARBOXYFLUORESCEIN (BCECF) [J].
KOLBER, MA ;
QUINONES, RR ;
GRESS, RE ;
HENKART, PA .
JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 108 (1-2) :255-264
[10]   NEUTROPHIL CHEMOTAXIS IN GASTRIC-MUCOSA - A SIGNAL-TO-RESPONSE COMPARISON [J].
KOZOL, R ;
DOMANOWSKI, A ;
JASZEWSKI, R ;
CZANKO, R ;
MCCURDY, B ;
PRASAD, M ;
FROMM, B ;
CALZADA, R .
DIGESTIVE DISEASES AND SCIENCES, 1991, 36 (09) :1277-1280