PKC-INDEPENDENT INHIBITION OF GLUTAMATE EXOCYTOSIS BY ARACHIDONIC-ACID IN RAT CEREBROCORTICAL SYNAPTOSOMES

被引：11

作者：

HERRERO, I ^{[1
]}

MIRASPORTUGAL, MT ^{[1
]}

SANCHEZPRIETO, J ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT BIOQUIM,E-28040 MADRID,SPAIN

来源：

FEBS LETTERS | 1992年 / 296卷 / 03期

关键词：

PROTEIN KINASE-C; GLUTAMATE EXOCYTOSIS; ARACHIDONIC ACID; SYNAPTOSOME; RAT BRAIN;

D O I：

10.1016/0014-5793(92)80312-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In rat cerebrocortical synaptosomes, the addition of 4-beta-phorbol dibutyrate (4-beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, prevent the stimulatory effect of 4-beta-PDBu, but are without effect on the inhibitory action of arachidonic acid. Moreover, methyl arachidonate, which is not effective as a PKC activator, also strongly inhibits glutamate exocytosis. These results suggest that PKC is not involved in the inhibition of glutamate release by arachidonic acid.

引用

页码：317 / 319

页数：3

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