THE RIBOSOMAL INTERGENIC SPACER REGION - A TARGET FOR THE PCR BASED DIAGNOSIS OF TUBERCULOSIS

被引：26

作者：

GLENNON, M

SMITH, T

CORMICAN, M

NOONE, D

BARRY, T

MAHER, M

DAWSON, M

GILMARTIN, JJ

GANNON, F

机构：

[1] NATL UNIV IRELAND UNIV COLL GALWAY,DEPT CLIN MICROBIOL,GALWAY,IRELAND

[2] NATL UNIV IRELAND UNIV COLL GALWAY,DEPT RESP MED,GALWAY,IRELAND

来源：

TUBERCLE AND LUNG DISEASE | 1994年 / 75卷 / 05期

关键词：

D O I：

10.1016/0962-8479(94)90081-7

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Setting: DNA Diagnostics, The National Diagnostics Centre and University College Hospital, Galway, Republic of Ireland. Objective: To investigate the possibility of developing a DNA probe to distinguish the members of the Mycobacterium tuberculosis complex and develop an assay for the detection of M. tuberculosis in sputum. Design: The ribosomal intergenic spacer regions from the members of the M. tuberculosis complex were sequenced and analysed for the ability of this region to provide sequence diversity for DNA probe development. Results: The 100% sequence homology in this region precluded the development of a probe to distinguish the individual members of the M. tuberculosis complex. However it was possible to develop a DNA probe that could specifically detect only the members of the M. tuberculosis complex. Conclusion: A specific DNA probe was developed for the detection of the M. tuberculosis complex and its application to M. tuberculosis detection in sputum was demonstrated.

引用

页码：353 / 360

页数：8

共 22 条

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