T-CELL ACTIVATION IN HUMAN INTESTINAL-MUCOSA - THE ROLE OF SUPERANTIGENS

被引:34
作者
AISENBERG, J
EBERT, EC
MAYER, L
机构
[1] MT SINAI MED CTR,DIV CLIN IMMUNOL,NEW YORK,NY 10029
[2] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MED,NEW BRUNSWICK,NJ 08903
关键词
D O I
10.1016/0016-5085(93)90147-5
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Superantigens are a class of potent T-cell mitogens that activate T cells using specific antigen receptor V regions. Superantigens have been implicated in the pathogenesis of several autoimmune diseases, including inflammatory bowel disease. The present study examines the role of Superantigens in the human gastrointestinal immune response. Methods: Human intestinal epithelial cells and T lymphocytes were obtained from surgical specimens and cultured in the presence or absence of exogenous superantigens. Activation of T cells and V region usage were measured by thymidine incorporation and by cell staining using a panel of monoclonal antibodies. Results: Neither epithelial cells from normal nor diseased intestinal mucosa expressed virally encoded, endogenous superantigens. However, 50% of epithelial cell preparations could present exogenous bacterial superantigens to T cells. In the other 50%, a defect in this function was observed, which did not represent production of suppressive factors or absence of accessory cytokines. Mucosal T lymphocytes proliferated in response to superantigens in vitro, expressing increased transferrin receptor, interleukin-2 receptor, and HLA-DR. Conclusions: A superantigen-driven mucosal immune response may occur in health and in chronic inflammatory states. The intestinal epithelial cell may mediate this response, through presentation of superantigens to mucosal T lymphocytes. © 1992.
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页码:1421 / 1430
页数:10
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