DETERMINATION OF HETEROCYCLIC-COMPOUNDS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

被引：14

作者：

BJERGEGAARD, C ^{[1
]}

SIMONSEN, H ^{[1
]}

SORENSEN, H ^{[1
]}

机构：

[1] ROYAL VET & AGR UNIV,DEPT CHEM,DK-1871 FREDERIKSBERG,DENMARK

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)85155-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Micellar electrokinetic capillary chromatography (MECC) based on sodium cholate (NaCh) and sodium dodecyl sulphate (SDS) was developed for the determination of aromatic amino acids and heterocyclic legume constituents. The influence of temperature, voltage, micellar system, pH, zwitterion and modifier concentrations in the buffer on migration times, peak areas, resolution and number of theoretical plates was investigated. This MECC method makes possible the sensitive determination of the individual compounds with detection limits in the picomole range. Up to 300000 theoretical plates per metre of capillary were obtained together with satisfactory linearity and repeatability of the NaCh method. The applicability of MECC to samples prepared from plant material, following a fast and simple technique of isolation, purification and group separation, is illustrated by selected examples.

引用

页码：561 / 569

页数：9

共 23 条

[1] HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS FOR THE DETERMINATION OF TRYPSIN AND CHYMOTRYPSIN INHIBITORS AND THEIR ASSOCIATION WITH TRYPSIN, CHYMOTRYPSIN AND MONOCLONAL-ANTIBODIES [J].

ARENTOFT, AM ;

FROKIAER, H ;

MICHAELSEN, S ;

SORENSEN, H ;

SORENSEN, S .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (01) :189-198

[2] THE NUTRITIVE-VALUE OF 10 INBRED LINES OF FABA BEANS (VICIA-FABA L) IN RELATION TO THEIR CONTENT OF ANTINUTRITIONAL CONSTITUENTS AND PROTEIN-QUALITY [J].

BJERG, B ;

EBMEYER, E ;

EGGUM, BO ;

LARSEN, T ;

ROBBELEN, G ;

SORENSEN, H .

PLANT BREEDING, 1988, 101 (04) :277-291

[3]

BJERG B, 1985, Z PFLANZENZUCHT, V94, P135

[4]

BJERG B, 1984, Z TIERPHYSIOL TIERER, V51, P275

[5]

BJERG B, 1984, J PLANT DIS PROTECT, V9, P483

[6]

BJERG B, 1984, VICIA FABA AGRONOMY, V10, P287

[7] DETERMINATION OF FLAVONOIDS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].

BJERGEGAARD, C ;

MICHAELSEN, S ;

MORTENSEN, K ;

SORENSEN, H .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :477-485

[8] DETERMINATION OF PHENOLIC CARBOXYLIC-ACIDS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY AND EVALUATION OF FACTORS AFFECTING THE METHOD [J].

BJERGEGAARD, C ;

MICHAELSEN, S ;

SORENSEN, H .

JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :403-411

[9]

BJERGEGAARD C, 1990, J CHROMATOGR A, V653, P99

[10] SODIUM GLYCODEOXYCHOLATE AND TAURODEOXYCHOLATE - POSSIBLE HELICAL MODELS FOR CONJUGATED BILE-SALT MICELLES [J].

CAMPANELLI, AR ;

DESANCTIS, SC ;

CHIESSI, E ;

DALAGNI, M ;

GIGLIO, E ;

SCARAMUZZA, L .

JOURNAL OF PHYSICAL CHEMISTRY, 1989, 93 (04) :1536-1542

← 1 2 3 →