CONTINUOUS ANALYSIS OF THE MECHANISM OF ACTIVATED TRANSBILAYER LIPID MOVEMENT IN PLATELETS

被引：163

作者：

WILLIAMSON, P

BEVERS, EM

SMEETS, EF

COMFURIUS, P

SCHLEGEL, RA

ZWAAL, RFA

机构：

[1] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT BIOCHEM,6200 MD MAASTRICHT,NETHERLANDS

[2] AMHERST COLL,DEPT BIOL,AMHERST,MA 01002

[3] PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,UNIVERSITY PK,PA 16802

来源：

BIOCHEMISTRY | 1995年 / 34卷 / 33期

关键词：

D O I：

10.1021/bi00033a017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dithionite reduction of fluorescent (NBD) phospholipids was used as the basis of a continuous assay of transbilayer lipid movement to the cell surface during platelet activation. This assay reveals that virtually all previously internalized phosphatidylserine passes through the external leaflet of the membrane within 90s after activation with Ca2+ and ionophore or with thrombin and thapsigargin. We demonstrate that this lipid scrambling is reversible, bidirectional, and insensitive to the lipid headgroup. Prolonged activation gradually results in inactivation of the scramblase. The assay also reveals that activation of the scrambling activity is sensitive to the sulfhydryl reagent pyridyldithioethylamine, suggesting the involvement of a protein in the process of activated transbilayer lipid scrambling.

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页码：10448 / 10455

页数：8

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