QUANTITATION OF DERMATAN SULFATE ACTIVE-SITE FOR HEPARIN-COFACTOR-II BY H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY

被引：20

作者：

MASCELLANI, G ^{[1
]}

LIVERANI, L ^{[1
]}

PRETE, A ^{[1
]}

BERGONZINI, G ^{[1
]}

TORRI, G ^{[1
]}

BISIO, A ^{[1
]}

GUERRINI, M ^{[1
]}

CASU, B ^{[1
]}

BIANCHINI, P ^{[1
]}

机构：

[1] IST CHIM & BIOCHIM G RONZONI, MILAN, ITALY

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 223卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1558

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence (IdoA2SO(3)-GalNAc4SO(3))(n) contributes to the HCII-mediated inhibition of thrombin by dermatan sulfate (DS). This sequence clearly results from the C-13 NMR spectrum and can be quantified by the signal C1-H of IdoA2SO(3) in the H-1 NMR spectrum. A linear correlation has been found between the content in the disulfated disaccharide Delta Di-2,4diS obtained by enzymatic demolition with ABC lyase, the percentage content in ldoA2SO(3) quantified by H-1 NMR, and the HCII-mediated activity of dermatan sulfates from beef mucosa and pig skin. DSs have been obtained also from pig mucosa and contain an amount, not negligible, of Delta Di-4,6diS. This disulfate disaccharide contributes to the activity expressed by the IdoA2SO(3)-GalNAc4SO(3) sequence. The analytical techniques HPLC and H-1 NMR, applied to the currently performed analyses of DS, are described and discussed. (C) 1994 Academic Press,Inc.

引用

页码：135 / 141

页数：7

共 16 条

[1] FRACTIONATION AND IDENTIFICATION OF HEPARIN AND OTHER ACIDIC MUCOPOLYSACCHARIDES BY A NEW DISCONTINUOUS ELECTROPHORETIC METHOD [J].