POSSIBILITIES OF CELL-FUSION AND TRANSFORMATION BY ELECTROSTIMULATION

被引：27

作者：

BERG, H

AUGSTEN, K

BAUER, E

FORSTER, W

JACOB, HE

MUHLIG, P

WEBER, H

机构：

[1] Academy of Sciences of the G.D.R., Central Institute of Microbiology and Experimental Therapy, Department of Biophysical Chemistry, Jena, German Democratic Republic

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1984年 / 12卷 / 1-2期

关键词：

ELECTROCHEMISTRY; -; MEMBRANES; YEAST;

D O I：

10.1016/0302-4598(84)85155-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A survey of three electrostimulation techniques: (A) the micro-technique with agglutination of cells, (B) the micro-technique with dielectrophoretic collection of cells, and (C) the micromanipulator technique with needle electrodes for contacting cells. These are illustrated by examples of fusion between protoplasts of microbiological mutants, or between animal cells on the one hand and the enhancement of the transformation rate with plasmid DNA on the other. For an explanation of pore enlargement and membrane breakdown, an electrodichroitic model is described. From these results is appears that this new bioelectrochemical method will become a powerful tool for gene- and biotechnology.

引用

页码：119 / 133

页数：15

共 29 条

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