HIGHLY BIOLUMINESCENT BACILLUS-SUBTILIS OBTAINED THROUGH HIGH-LEVEL EXPRESSION OF A LUXAB FUSION GENE

被引:20
作者
JACOBS, M
HILL, PJ
STEWART, GSAB
机构
[1] UNIV NOTTINGHAM,FAC AGR & FOOD SCI,DEPT APPL BIOCHEM & FOOD SCI,LOUGHBOROUGH LE12 5RD,LEICS,ENGLAND
[2] TECH UNIV DENMARK,DEPT MICROBIOL,DK-2800 LYNGBY,DENMARK
来源
MOLECULAR & GENERAL GENETICS | 1991年 / 230卷 / 1-2期
关键词
BACILLUS-SUBTILIS; LUCIFERASE; BIOLUMINESCENCE; TRANSLATIONAL COUPLING;
D O I
10.1007/BF00290675
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bioluminescence levels comparable to those achievable in Escherichia coli have yet to be obtained from luxAB expression in gram-positive bacteria. In this communication we describe the gene engineering required to generate a highly bioluminescent derivative of Bacillus subtilis. The combination of a powerful promoter, P(xyn), a fusion derivative of luxAB from Vibrio harveyi and translational coupling have overcome the previously reported limitations in luxAB expression. The implications for highly bioluminescent gram-positive organisms are discussed.
引用
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页码:251 / 256
页数:6
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