COMBINED LIQUID-CHROMATOGRAPHY ELECTRON-PARAMAGNETIC RESONANCE SPECTROMETRY ELECTROSPRAY IONIZATION MASS-SPECTROMETRY FOR RADICAL IDENTIFICATION

被引：55

作者：

IWAHASHI, H ^{[1
]}

PARKER, CE ^{[1
]}

MASON, RP ^{[1
]}

TOMER, KB ^{[1
]}

机构：

[1] NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 19期

关键词：

D O I：

10.1021/ac00043a011

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electron paramagnetic resonance (EPR) spectrometry and mass spectrometry (MS) have been coupled together on-line with liquid chromatography (LC)/UV detection. These combined techniques have been applied to the determination of spin-trapped radical adducts, including phenyl, 2-, 3-, and 4- chlorophenyl, and 2-bromophenyl radicals trapped with alpha-(1-oxo-4-pyridyl)-N-tert-butyinitrone (4-POBN), and phenyl radicals trapped with 2-methyl-2-nitrosopropane (MNP), alpha-phenyl-N-tert-butyinitrone (PBN), and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Oxidized and reduced forms of the radical adducts were also detected by the on-line LC/EPR/Ms system.

引用

页码：2244 / 2252

页数：9

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