SEQUENCE-ANALYSIS OF THE MEMBRANE-PROTEIN GENE OF HUMAN CORONAVIRUS OC43 AND EVIDENCE FOR O-GLYCOSYLATION

被引:51
作者
MOUNIR, S [1 ]
TALBOT, PJ [1 ]
机构
[1] UNIV QUEBEC,INST ARMAND FRAPPIER,VIROL RES CTR,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
关键词
D O I
10.1099/0022-1317-73-10-2731
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The gene encoding the membrane (M) protein of the OC43 strain of human coronavirus (HCV-OC43) was amplified by a reverse transcription-polymerase chain reaction of viral RNA with HCV-OC43- and bovine coronavirus (BCV)-specific primers. The nucleotide sequence of the cloned 1-5 kb fragment revealed an open reading frame (ORF) of 690 nucleotides which was identified as the M protein gene from its homology to BCV. This ORF encodes a protein of 230 amino acids with an M(r) of 26416. The gene is preceded by the motif UCCAAAC, analogous to the consensus coronavirus transcription initiation sequence. The M protein of HCV-OC43 shows features typical of all coronavirus M proteins studied: a hydrophilic, presumably external N terminus including about 10% of the protein, and a potential N-glycosylation site followed by three major hydrophobic transmembrane domains. The amino acid sequence of the M protein of HCV-OC43 has 94% identity with that of the Mebus strain of BCV, and also contains six potential O-glycosylation sites in the exposed N-terminal domain. Indeed, the glycosylation of the M protein was not inhibited in the presence of tunicamycin, which is indicative of O-glycosylation, as previously reported for BCV and murine hepatitis virus. Virions released from tunicamycin-treated cells contained the M glycoprotein but were devoid of both peplomer (S) and haemagglutinin-esterase (HE) proteins. Thus, inhibition of the N-glycosylation of the S and HE structural proteins prevented their incorporation into progeny virions, an indication that they are dispensable for virion morphogenesis, unlike the M protein.
引用
收藏
页码:2731 / 2736
页数:6
相关论文
共 40 条
  • [21] SEQUENCE AND N-TERMINAL PROCESSING OF THE TRANSMEMBRANE PROTEIN-E1 OF THE CORONAVIRUS TRANSMISSIBLE GASTROENTERITIS VIRUS
    LAUDE, H
    RASSCHAERT, D
    HUET, JC
    [J]. JOURNAL OF GENERAL VIROLOGY, 1987, 68 : 1687 - 1693
  • [22] THE COMPLETE SEQUENCE (22 KILOBASES) OF MURINE CORONAVIRUS GENE-1 ENCODING THE PUTATIVE PROTEASES AND RNA-POLYMERASE
    LEE, HJ
    SHIEH, CK
    GORBALENYA, AE
    KOONIN, EV
    LAMONICA, N
    TULER, J
    BAGDZHADZHYAN, A
    LAI, MMC
    [J]. VIROLOGY, 1991, 180 (02) : 567 - 582
  • [23] McINTOSH K., 1974, Current Topics in Microbiology and Immunology, P85
  • [24] CORONAVIRUS GLYCOPROTEIN-E1, A NEW TYPE OF VIRAL GLYCOPROTEIN
    NIEMANN, H
    KLENK, HD
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1981, 153 (04) : 993 - 1010
  • [25] SEQUENCE-ANALYSIS REVEALS EXTENSIVE POLYMORPHISM AND EVIDENCE OF DELETIONS WITHIN THE E2-GLYCOPROTEIN GENE OF SEVERAL STRAINS OF MURINE HEPATITIS-VIRUS
    PARKER, SE
    GALLAGHER, TM
    BUCHMEIER, MJ
    [J]. VIROLOGY, 1989, 173 (02) : 664 - 673
  • [26] ANTIGENIC RELATIONSHIP OF FELINE INFECTIOUS PERITONITIS VIRUS TO CORONAVIRUSES OF OTHER SPECIES
    PEDERSEN, NC
    WARD, J
    MENGELING, WL
    [J]. ARCHIVES OF VIROLOGY, 1978, 58 (01) : 45 - 53
  • [27] CORONAVIRUS MHV-JHM - NUCLEOTIDE-SEQUENCE OF THE MESSENGER-RNA THAT ENCODES THE MEMBRANE-PROTEIN
    PFLEIDERER, M
    SKINNER, MA
    SIDDELL, SG
    [J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (15) : 6338 - 6338
  • [28] PORCINE RESPIRATORY CORONAVIRUS DIFFERS FROM TRANSMISSIBLE GASTROENTERITIS VIRUS BY A FEW GENOMIC DELETIONS
    RASSCHAERT, D
    DUARTE, M
    LAUDE, H
    [J]. JOURNAL OF GENERAL VIROLOGY, 1990, 71 : 2599 - 2607
  • [29] ISOLATION AND PROPAGATION OF A HUMAN ENTERIC CORONAVIRUS
    RESTA, S
    LUBY, JP
    ROSENFELD, CR
    SIEGEL, JD
    [J]. SCIENCE, 1985, 229 (4717) : 978 - 981
  • [30] PREDICTED MEMBRANE TOPOLOGY OF THE CORONAVIRUS PROTEIN-E1
    ROTTIER, PJM
    WELLING, GW
    WELLINGWESTER, S
    NIESTERS, HGM
    LENSTRA, JA
    VANDERZEIJST, BAM
    [J]. BIOCHEMISTRY, 1986, 25 (06) : 1335 - 1339