SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION - THE CRYSTAL-STRUCTURE OF HOECHST-33258 BOUND TO THE D(CGCAAATTTGCG)(2) DUPLEX

被引：118

作者：

SPINK, N ^{[1
]}

BROWN, DG ^{[1
]}

SKELLY, JV ^{[1
]}

NEIDLE, S ^{[1
]}

机构：

[1] INST CANC RES,CRC,BIOMOLEC STRUCT UNIT,SUTTON SM2 5NG,SURREY,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 09期

关键词：

D O I：

10.1093/nar/22.9.1607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)(2). The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)(2). Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.

引用

页码：1607 / 1612

页数：6

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