SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION - THE CRYSTAL-STRUCTURE OF HOECHST-33258 BOUND TO THE D(CGCAAATTTGCG)(2) DUPLEX

被引：118

作者：

SPINK, N ^{[1
]}

BROWN, DG ^{[1
]}

SKELLY, JV ^{[1
]}

NEIDLE, S ^{[1
]}

机构：

[1] INST CANC RES,CRC,BIOMOLEC STRUCT UNIT,SUTTON SM2 5NG,SURREY,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 09期

关键词：

D O I：

10.1093/nar/22.9.1607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)(2). The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)(2). Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.

引用

页码：1607 / 1612

页数：6

共 30 条

[11] INTERACTION OF HOECHST 33258 WITH THE MINOR GROOVE OF THE A+T-RICH DNA DUPLEX D(GGTAATTACC)2 STUDIED IN SOLUTION BY NMR-SPECTROSCOPY [J].

EMBREY, KJ ;

SEARLE, MS ;

CRAIK, DJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 211 (03) :437-447

[12] DETERMINATION OF THE NMR SOLUTION STRUCTURE OF THE HOECHST 33258-D(GTGGAATTCCAC)(2) COMPLEX AND COMPARISON WITH THE X-RAY CRYSTAL-STRUCTURE [J].

FEDE, A ;

BILLETER, M ;

LEUPIN, W ;

WUTHRICH, K .

STRUCTURE, 1993, 1 (03) :177-186

[13] DYNAMICS AND BINDING MODE OF HOECHST 33258 TO D(GTGGAATTCCAC)2 IN THE 1-1 SOLUTION COMPLEX AS DETERMINED BY 2-DIMENSIONAL H-1-NMR [J].

FEDE, A ;

LABHARDT, A ;

BANNWARTH, W ;

LEUPIN, W .

BIOCHEMISTRY, 1991, 30 (48) :11377-11388

[14] ISOHELICAL ANALYSIS OF DNA GROOVE-BINDING DRUGS [J].

GOODSELL, D ;

DICKERSON, RE .

JOURNAL OF MEDICINAL CHEMISTRY, 1986, 29 (05) :727-733

[15] MOLECULAR RECOGNITION OF B-DNA BY HOECHST 33258+ [J].

HARSHMAN, KD ;

DERVAN, PB .

NUCLEIC ACIDS RESEARCH, 1985, 13 (13) :4825-4835

[16]

KOPKA ML, 1992, NUCLEIC ACID TARGETE, P304

[17]

LOEWE H, 1974, ARZNEIMITTELFORSCH, V24, P1927

[18] SEQUENCE SPECIFICITY OF I-125-LABELED HOECHST-33258 DAMAGE IN 6 CLOSELY RELATED DNA-SEQUENCES [J].

MURRAY, V ;

MARTIN, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (01) :63-73

[19] MINOR-GROOVE RECOGNITION OF THE SELF-COMPLEMENTARY DUPLEX D(CGCGAATTCGCG)2 BY HOECHST-33258 - A HIGH-FIELD NMR-STUDY [J].

PARKINSON, JA ;

BARBER, J ;

DOUGLAS, KT ;

ROSAMOND, J ;

SHARPLES, D .

BIOCHEMISTRY, 1990, 29 (44) :10181-10190

[20] BINDING OF HOECHST-33258 TO THE MINOR GROOVE OF B-DNA [J].

PJURA, PE ;

GRZESKOWIAK, K ;

DICKERSON, RE .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 197 (02) :257-271

← 1 2 3 →