ONCHOCERCA-VOLVULUS, O-GUTTUROSA, BRUGIA-MALAYI, AND DIROFILARIA-IMMITIS - A COMPARATIVE-STUDY OF THE IMMUNOCHEMICAL PROPERTIES OF CUTICULAR PROTEINS FROM FILARIAL PARASITES

被引：10

作者：

PETRALANDA, I ^{[1
]}

PIESSENS, WF ^{[1
]}

机构：

[1] CTR AMAZON INVEST & CONTROL ENFERMEDADES TROP, MINIST SANIDAD & ASISTENC SOCIAL, PUERTO AYACUCHO 7101, VENEZUELA

来源：

EXPERIMENTAL PARASITOLOGY | 1991年 / 72卷 / 02期

关键词：

ONCHOCERCA-VOLVULUS; O-GUTTUROSA; BRUGIA-MALAYI; DIROFILARIA-IMMITIS; FILARIA; NEMATODE; CUTICLE; ANTIGEN; COLLAGEN; MICROFILARIAE (MF); ADULT WORM (AW); INFECTIVE (3RD STAGE) LARVAE (L3); ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA); SODIUM DODECYL SULFATE (SDS); POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE); PHOSPHATE-BUFFERED SALINE (PBS); CUTICULAR PROTEINS (CP); TRICHLOROACETIC ACID (TCA); 2-MERCAPTOETHANOL (2-ME);

D O I：

10.1016/0014-4894(91)90134-I

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We compared the chemical and immunological properties of cuticular collagens from four species of filarial nematodes, Onchocerca volvulus, O. gutturosa, Brugia malayi, and Dirofilaria immitis. The electrophoretic mobility of the major polypeptides extracted from adult worms is characteristic for each species studied. Cuticular collagens from adult worms and infective larvae differ in their susceptibility to proteases that cleave vertebrate collagens and to collagenases prepared from different developmental stages of filarial parasites. The overall amino acid composition of filarial collagens resembles that of vertebrate interstitial collagens and differs from that reported for collagens from free-living or intestinal nematodes. However, cuticular proteins of the four filarial species studied significantly differed in amino acid composition and in their reactivity with antisera to interstitial and basement membrane collagens of vertebrates. © 1991.

引用

页码：164 / 173

页数：10

共 39 条

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FORSYTH KP, 1981, ACTA TROP, V38, P329

[12]

FRANZ M, 1980, TROPENMED PARASITOL, V31, P149

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