PRODUCTION, ISOLATION AND CHARACTERIZATION OF HUMAN PROFILIN FROM SACCHAROMYCES-CEREVISIAE

被引:11
作者
ASPENSTROM, P
LASSING, I
KARLSSON, R
机构
[1] UNIV UPPSALA,CTR BIOMED,DEPT DEV BIOL,BOX 587,S-75123 UPPSALA,SWEDEN
[2] UNIV STOCKHOLM,ARRHENIUS LABS E5,DEPT ZOOL CELLBIOL,S-10691 STOCKHOLM,SWEDEN
关键词
D O I
10.1007/BF01774039
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.
引用
收藏
页码:201 / 207
页数:7
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