PRODUCTION, ISOLATION AND CHARACTERIZATION OF HUMAN PROFILIN FROM SACCHAROMYCES-CEREVISIAE

被引：11

作者：

ASPENSTROM, P

LASSING, I

KARLSSON, R

机构：

[1] UNIV UPPSALA,CTR BIOMED,DEPT DEV BIOL,BOX 587,S-75123 UPPSALA,SWEDEN

[2] UNIV STOCKHOLM,ARRHENIUS LABS E5,DEPT ZOOL CELLBIOL,S-10691 STOCKHOLM,SWEDEN

来源：

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY | 1991年 / 12卷 / 02期

关键词：

D O I：

10.1007/BF01774039

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.

引用

页码：201 / 207

页数：7

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