POSITIONAL CLONING AND SEQUENCE-ANALYSIS OF THE DROSOPHILA CLOCK GENE, TIMELESS

被引：210

作者：

MYERS, MP

WAGERSMITH, K

WESLEY, CS

YOUNG, MW

SEHGAL, A

机构：

[1] ROCKEFELLER UNIV, CTR BIOL TIMING, HOWARD HUGHES MED INST, NATL SCI FDN SCI & TECHNOL, NEW YORK, NY 10021 USA

[2] ROCKEFELLER UNIV, GENET LAB, NEW YORK, NY 10021 USA

[3] UNIV PENN, SCH MED, DEPT NEUROSCI, PHILADELPHIA, PA 19104 USA

[4] UNIV PENN, SCH MED, CTR SLEEP & RESP NEUROBIOL, PHILADELPHIA, PA 19104 USA

来源：

SCIENCE | 1995年 / 270卷 / 5237期

关键词：

D O I：

10.1126/science.270.5237.805

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Drosophila genes timeless (tim) and period (per) interact, and both are required for production of circadian rhythms. Here the positional cloning and sequencing of tim are reported. The tim gene encodes a previously uncharacterized protein of 1389 amino acids, and possibly another protein of 1122 amino acids. The arrhythmic mutation tim(01) is a 64-base pair deletion that truncates TIM to 749 amino acids. Absence of sequence similarity to the PER dimerization motif (PAS) indicates that direct interaction between PER and TIM would require a heterotypic protein association.

引用

页码：805 / 808

页数：4

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