EVIDENCE FOR A FUNCTIONAL GLUCOCORTICOID RESPONSIVE ELEMENT IN THE EPSTEIN-BARR-VIRUS GENOME

被引：15

作者：

SCHUSTER, C

CHASSEROTGOLAZ, S

URIER, G

BECK, G

SERGEANT, A

机构：

[1] CNRS,INST BIOL MOLEC & CELLULAIRE,15 RUE RENE DESCARTES,F-67084 STRASBOURG,FRANCE

[2] ECOLE NORM SUPER,VIROL MOLEC LAB,F-69364 LYONS 07,FRANCE

来源：

MOLECULAR ENDOCRINOLOGY | 1991年 / 5卷 / 02期

关键词：

D O I：

10.1210/mend-5-2-267

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Glucocorticoids induce the expression of Epstein-Barr virus early antigens in latently infected Daudi cells. By sequence analysis, we found that fragment C of the BamHl digested Epstein-Barr virus B95-8 genome contains a region with a large degree of homology to the glucocorticoid responsive element of known glucocorticoid-regulated genes. By transfection experiments in Daudi and HeLa cells, different lengths of this region, cloned in front of the bacterial chloramphenicol acetyl transferase linked to the Herpes Simplex virus thymidine kinase promoter (pBLCAT.2), were assayed for their responsiveness to dexamethasone; our results led us to the conclusion that the hormonal effect observed was mediated by a minimal sequence of 15 base pairs presenting 85% homology with the consensus glucocorticoid responsive element sequence.

引用

页码：267 / 272

页数：6

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[11] THE UPSTREAM REGULATORY REGION OF THE HUMAN PAPILLOMA VIRUS-16 CONTAINS AN E2-PROTEIN-INDEPENDENT ENHANCER WHICH IS SPECIFIC FOR CERVICAL-CARCINOMA CELLS AND REGULATED BY GLUCOCORTICOID HORMONES [J].