IDENTIFICATION OF THE MAJOR IMMUNOGENIC STRUCTURAL PROTEINS OF HUMAN FOAMY VIRUS

被引：48

作者：

NETZER, KO ^{[1
]}

RETHWILM, A ^{[1
]}

MAURER, B ^{[1
]}

TERMEULEN, V ^{[1
]}

机构：

[1] UNIV WURZBURG,INST VIROL & IMMUNBIOL,VERSBACHER STR 7,W-8700 WURZBURG,GERMANY

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-5-1237

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have identified the major immunogenic structural proteins of the human foamy virus (HFV), a distinct member of the foamy virus subfamily of Retroviridae. Radiolabelled viral proteins were immunoprecipitated from HFV-infected cells by foamy virus antisera of human and non-human primate origin. Precipitated viral proteins were in the range of 31K to 170K. Labelling of proteins with [14C]glucosamine or with [35S]methionine in the presence of tunicamycin, as well as endo-β-N-acetylglycosaminidase H and F treatment of [35S]methionine-labelled proteins, revealed three viral glycoproteins of approximately 170K, 130K and 47K, most likely representing the env gene-encoded precursor, the surface glycoprotein and the transmembrane protein of HFV, respectively.

引用

页码：1237 / 1241

页数：5