AFFINITY PURIFICATION AND CDNA CLONING OF RAT NEURAL DIFFERENTIATION AND TUMOR-CELL SURFACE-ANTIGEN GP130(RB13-6) REVEALS RELATIONSHIP TO HUMAN AND MURINE PC-1

被引:69
作者
DEISSLER, H
LOTTSPEICH, F
RAJEWSKY, MF
机构
[1] UNIV ESSEN GESAMTHSCH,SCH MED,INST CELL BIOL CANC RES,D-45122 ESSEN,GERMANY
[2] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY
关键词
D O I
10.1074/jbc.270.17.9849
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Monoclonal antibody RB13-6 recognizes a subset of rat brain glial precursor cells that are highly susceptible to malignant conversion by the carcinogen N-ethyl-N-nitrosourea. The corresponding cell surface antigen was identified as a membrane glycoprotein (gp130(RB13-6)) and purified by immunoaffinity chromatography from the tumorigenic neuroectodermal rat cell line BT4Ca, Sequencing of 5 endoproteinase generated peptides of the purified antigen permitted the specific amplification of a cDNA fragment by reverse transcription-polymerase chain reaction and subsequent isolation of the complete coding sequence from a fetal rat brain cDNA library, The derived amino acid sequence indicates that the RB13-6 antigen is related to the human and murine plasma cell membrane protein PC-1, a nucleotide pyrophosphatase/alkaline phosphodiesterase and ectoprotein kinase. Similarly, purified gp130(RB13-6) possesses 5'-nucleotidase activity that can be inhibited with EDTA. Different from PC-1, gp130(RB13-6) isolated from BT4Ca cells is not a disulfide-linked dimer and contains an RGD-tripeptide sequence which, together with other structural features, suggests a possible function in cell adhesion and its subversion in malignant phenotypes.
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页码:9849 / 9855
页数:7
相关论文
共 42 条
[41]   PHOSPHORYLATION AND NUCLEOTIDE-DEPENDENT DEPHOSPHORYLATION OF HEPATIC POLYPEPTIDES RELATED TO THE PLASMA-CELL DIFFERENTIATION ANTIGEN PC-1 [J].
URIARTE, M ;
STALMANS, W ;
HICKMAN, S ;
BOLLEN, M .
BIOCHEMICAL JOURNAL, 1993, 293 :93-100
[42]  
VANDRIEL IR, 1987, J BIOL CHEM, V262, P4882