MAPPING AND POSITIONING DNA-BINDING PROTEINS ALONG GENOMIC DNA - STRUCTURE OF DROSOPHILA-MELANOGASTER RIBOSOMAL ALU-REPEATS AND 1.688 SATELLITE CHROMATIN

被引：18

作者：

BELIKOV, SV ^{[1
]}

BELGOVSKY, AI ^{[1
]}

PARTOLINA, MP ^{[1
]}

KARPOV, VL ^{[1
]}

MIRZABEKOV, AD ^{[1
]}

机构：

[1] WA ENGELHARDT INST MOLEC BIOL, MOSCOW 117984, RUSSIA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 20期

关键词：

D O I：

10.1093/nar/21.20.4796

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin structure of so-called 'Alu-repeat' in D.melanogaster ribosomal non-transcribed spacer that contains sequences homologous to the promoter of ribosomal genes has been studied. Using the 'protein image' hybridization assay based on UV-light-induced DNA-protein crosslinking and 2-D gel retardation electrophoresis, two proteins of the molecular mass of 50 kD (rABP50) and 70 kD (rABP70), associated with 'Alu-repeat' DNA have been found. Exo III mapping of crosslinking sites and DNase I footprinting have provided a detailed map of H1, rABP50 and rABP70 contacts within the 'Alu-repeat' and H1 and a nonhistone protein contacts on satellite DNA. These data indicate precise positioning of non-histone proteins, histone Hl and nucleosomes within genomic regions studied and account for the presence of unusual 240 bp long nucleosomal particles in 'Alu-repeats'. The same approach can be adapted for succesive mapping and positioning proteins on genomic DNA.

引用

页码：4796 / 4802

页数：7