IMPROVED ELECTROCHEMICAL DETECTION OF DIURETICS IN HIGHPERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS BY POSTCOLUMN ONLINE PHOTOLYSIS

被引：19

作者：

MACHER, M ^{[1
]}

WINTERSTEIGER, R ^{[1
]}

机构：

[1] KARL FRANZENS UNIV GRAZ,INST PHARMACEUT CHEM,A-8010 GRAZ,AUSTRIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 709卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00440-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, high-performance liquid chromatographic method including postcolumn on-line UV irradiation and electrochemical detection is described for the determination of pharmaceuticals with diuretic action. The investigations on this liquid chromatography-photolysis-coulometric detection approach indicate that, depending on the substituents of the compounds, oxidation of drugs containing an aromatic chloride and thiazide moiety occurs at potentials as low as +200 mV. Parameters influencing the electrochemical response are given to optimize the reaction conditions. The procedure presented allows the quantification of diuretics, and consequently of chemically related substances, with good reproducibility and high selectivity at the picogram level.

引用

页码：257 / 264

页数：8

共 21 条

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