CORRECTION OF A DEFECT IN MAMMALIAN GPI ANCHOR BIOSYNTHESIS BY A TRANSFECTED YEAST GENE

被引：88

作者：

DEGASPERI, R

THOMAS, LJ

SUGIYAMA, E

CHANG, HM

BECK, PJ

ORLEAN, P

ALBRIGHT, C

WANECK, G

SAMBROOK, JF

WARREN, CD

YEH, ETH

机构：

[1] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT MED,ARTHRIT UNIT,BOSTON,MA 02114

[2] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235

[3] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02114

[4] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT ANESTHESIA,BOSTON,MA 02114

[5] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT SURG,BOSTON,MA 02114

[6] MIT,CTR CANC RES,CAMBRIDGE,MA 02139

来源：

SCIENCE | 1990年 / 250卷 / 4983期

关键词：

D O I：

10.1126/science.1978413

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.

引用

页码：988 / 991

页数：4