A BOVINE IGG HEAVY-CHAIN CONTAINS N-ACETYLGALACTOSAMINYLATED N-LINKED SUGAR CHAINS

被引:9
作者
AOKI, N
FURUKAWA, K
IWATSUKI, K
NODA, A
SATO, T
NAKAMURA, R
MATSUDA, T
机构
[1] NAGOYA UNIV,SCH AGR SCI,DEPT APPL BIOL SCI,NAGOYA,AICHI 46401,JAPAN
[2] TOKYO METROPOLITAN GERIATR HOSP & INST GERENTOL,DEPT BIOSIGNAL RES,ITABASHI KU,TOKYO 173,JAPAN
[3] UNIV TOKYO,INST MED SCI,DEPT BIOCHEM,TOKYO 108,JAPAN
关键词
D O I
10.1006/bbrc.1995.1657
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 56K protein co-purified with bovine milk fat globule membrane (MF-GM) proteins bound to Wisteria floribunda agglutinin (WFA) like most MFGM glycoproteins. Treatment with N-glycanase or beta-N-acetylhexosaminidase abolished the lectin binding to the protein. Amino acid sequence and immunoblot analyses revealed that the 56K protein is an IgG heavy chain. Lectin column chromatography of the oligosaccharides released by hydrazinolysis from the purified IgG heavy chains revealed that 0.08% of the total N-linked sugar chains bind to a WFA-agarose column, suggesting that they contain the beta-N-acetylgalactosaminylated structure. (C) 1995 Academic Press, Inc.
引用
收藏
页码:275 / 280
页数:6
相关论文
共 23 条
[11]   ASSOCIATION OF RHEUMATOID-ARTHRITIS AND PRIMARY OSTEO-ARTHRITIS WITH CHANGES IN THE GLYCOSYLATION PATTERN OF TOTAL SERUM IGG [J].
PAREKH, RB ;
DWEK, RA ;
SUTTON, BJ ;
FERNANDES, DL ;
LEUNG, A ;
STANWORTH, D ;
RADEMACHER, TW ;
MIZUOCHI, T ;
TANIGUCHI, T ;
MATSUTA, K ;
TAKEUCHI, F ;
NAGANO, Y ;
MIYAMOTO, T ;
KOBATA, A .
NATURE, 1985, 316 (6027) :452-457
[12]   MOST BOVINE-MILK FAT GLOBULE-MEMBRANE GLYCOPROTEINS CONTAIN ASPARAGINE-LINKED SUGAR CHAINS WITH GALNAC-BETA-1-]4GLCNAC GROUPS [J].
SATO, T ;
FURUKAWA, K ;
GREENWALT, DE ;
KOBATA, A .
JOURNAL OF BIOCHEMISTRY, 1993, 114 (06) :890-900
[13]   TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA [J].
SCHAGGER, H ;
VONJAGOW, G .
ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :368-379
[14]   STRUCTURE OF BOVINE IMMUNOGLOBULIN CONSTANT REGION HEAVY-CHAIN GAMMA-1 AND GAMMA-2 GENES [J].
SYMONS, DBA ;
CLARKSON, CA ;
BEALE, D .
MOLECULAR IMMUNOLOGY, 1989, 26 (09) :841-850
[15]   ASPARAGINE-LINKED OLIGOSACCHARIDE CHAINS OF IGG - REVISED STRUCTURE [J].
TAI, T ;
ITO, S ;
YAMASHITA, K ;
MURAMATSU, T ;
KOBATA, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 65 (03) :968-974
[16]  
TAKASAKI S, 1982, METHOD ENZYMOL, V83, P263
[17]   STRUCTURES OF THE SUGAR CHAINS OF RABBIT IMMUNOGLOBULIN-G - OCCURRENCE OF ASPARAGINE-LINKED SUGAR CHAINS IN FAB FRAGMENT [J].
TANIGUCHI, T ;
MIZUOCHI, T ;
BEALE, M ;
DWEK, RA ;
RADEMACHER, TW ;
KOBATA, A .
BIOCHEMISTRY, 1985, 24 (20) :5551-5557
[18]   ISOLATION AND PHYSICOCHEMICAL CHARACTERIZATION OF BOVINE SERUM AND COLOSTRAL IMMUNOGLOBULIN-G (IGG) SUBCLASSES [J].
TEWARI, UJ ;
MUKKUR, TKS .
IMMUNOCHEMISTRY, 1975, 12 (12) :925-930
[19]   GLYCOLIPID LECTIN INTERACTIONS - REACTIVITY OF LECTINS FROM HELIX-POMATIA, WISTERIA-FLORIBUNDA, AND DOLICHOS-BIFLORUS WITH GLYCOLIPIDS CONTAINING N-ACETYLGALACTOSAMINE [J].
TORRES, BV ;
MCCRUMB, DK ;
SMITH, DF .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 262 (01) :1-11
[20]   ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].
TOWBIN, H ;
STAEHELIN, T ;
GORDON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354