HOST-RANGE AND SEQUENCE-ANALYSIS OF AN ISOLATE OF POTATO-VIRUS-Y INDUCING VENOUS NECROSIS IN PEPPER

被引:13
作者
DAQUINO, L [1 ]
DALMAY, T [1 ]
BURGYAN, J [1 ]
RAGOZZINO, A [1 ]
SCALA, F [1 ]
机构
[1] UNIV NAPLES, IST PATOL VEGETALE, I-80055 PORTICI, ITALY
关键词
PVY COAT PROTEIN SEQUENCE; PVY 3' NONCODING REGION SEQUENCE; PVY TAXONOMY;
D O I
10.1094/PD-79-1046
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An isolate of potato virus Y (PVY), obtained from pepper (Capsicum annuum L.) showing veinal necrosis and named PVY-nnp, was compared with other PVY isolates for host range. In addition, the 3' noncoding region (3'-NCR) and coat protein gene (CP-gene) of PVY-nnp were cloned, sequenced, and aligned with the same regions of other PVY isolates. Biological characterization showed that PVY-nnp differs from all other PVY strains including isolates commonly infecting pepper. The nucleotide sequence comparison of PVY-nnp with other PVY isolates exhibited a homology ranging from 85.5 to 99.7% for the 3'-NCR, and from 87.5 to 94% for the CP-gene. The deduced amino acid sequence of the CP had a similarity ranging from 94.7 to 97.3%. These results indicate that PVY-nnp is a PVY isolate in spite of its peculiar biological properties.
引用
收藏
页码:1046 / 1050
页数:5
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