QUANTITATION OF MALARIA SPOROZOITES IN THE SALIVARY-GLANDS OF WILD AFROTROPICAL ANOPHELES

被引：33

作者：

BEIER, JC

ONYANGO, FK

RAMADHAN, M

KOROS, JK

ASIAGO, CM

WIRTZ, RA

KOECH, DK

ROBERTS, CR

机构：

[1] Kenya Medical Research Institute, U.S. Army Medical Research Unit, Nairobi

[2] Department of Entomology, Walter Reed Army Institute of Research, Washington, District of Columbia

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1991年 / 5卷 / 01期

关键词：

MALARIA; SPOROZOITES; QUANTITATION; ELISA; ANOPHELES-GAMBIAE; ANOPHELES-FUNESTUS; KENYA;

D O I：

10.1111/j.1365-2915.1991.tb00522.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).

引用

页码：63 / 70

页数：8

共 31 条

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