Vagal innervation of the thymus was studied by means of electrophysiological technique in the rat, Under urethane anesthesia, evoked action potentials originated from cervical vagus by electrical stimulations were recorded from the central cut end of the thymic branch of the vagus nerve after averaging for 32 times, It was observed that the conduction velocities are distributed in the range of 0.56 similar to 6.84 m/s, and the majority of vagal fibers in the thymic branch of the vagus nerve belong to a nonmyelinated C-fiber group, Further, it was confirmed that the right and left lobes of the thymus are innervated by cervical vagi bilaterally, The results suggest that the thymic branch of the vagus nerve plays a role in modulation of thymic function.