Crystallization and preliminary X-ray analysis of Borrelia burgdorferi outer surface protein A (OspA) complexed with a murine monoclonal antibody fab fragment

被引：15

作者：

Li, H ^{[1
]}

Lawson, CL ^{[1
]}

机构：

[1] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1995年 / 115卷 / 03期

关键词：

D O I：

10.1006/jsbi.1995.1058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Borrelia burgdorferi outer surface lipoprotein OspA is a current focus for vaccine development to prevent Lyme disease infection. A soluble, recombinant form of the protein lacking the amino-terminal lipid membrane anchor was cocrystallized with the Fab fragment of an agglutinating mouse monoclonal antibody. The crystals belong to space group P2(1)2(1)2(1), with a = 90.0 Angstrom, b = 91.9 Angstrom, and c = 102.9 Angstrom and they were found to diffract to a maximum resolution of 2.8 Angstrom using synchrotron radiation. (C) 1995 Academic Press, Inc.

引用

页码：335 / 337

页数：3

共 20 条

[1] BARGSTROM S, 1989, MOL MICROBIOL, V3, P479
[2] BOTT RR, 1982, J BIOL CHEM, V257, P9883
[3] IMMUNOGENIC INTEGRAL MEMBRANE-PROTEINS OF BORRELIA-BURGDORFERI ARE LIPOPROTEINS
BRANDT, ME
RILEY, BS
RADOLF, JD
NORGARD, MV
[J]. INFECTION AND IMMUNITY, 1990, 58 (04) : 983 - 991
[4] BORRELIA-BURGDORFERI BINDS PLASMINOGEN, RESULTING IN ENHANCED PENETRATION OF ENDOTHELIAL MONOLAYERS
COLEMAN, JL
SELLATI, TJ
TESTA, JE
KEW, RR
FURIE, MB
BENACH, JL
[J]. INFECTION AND IMMUNITY, 1995, 63 (07) : 2478 - 2484
[5] Dunn J J, 1990, Protein Expr Purif, V1, P159, DOI 10.1016/1046-5928(90)90011-M
[6] BORRELIA-BURGDORFERI IS CLONAL - IMPLICATIONS FOR TAXONOMY AND VACCINE DEVELOPMENT
DYKHUIZEN, DE
POLIN, DS
DUNN, JJ
WILSKE, B
PREACMURSIC, V
DATTWYLER, RJ
LUFT, BJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) : 10163 - 10167
[7] ROLE OF ATTACHED LIPID IN IMMUNOGENICITY OF BORRELIA-BURGDORFERI OSPA
ERDILE, LF
BRANDT, MA
WARAKOMSKI, DJ
WESTRACK, GJ
SADZIENE, A
BARBOUR, AG
MAYS, JP
[J]. INFECTION AND IMMUNITY, 1993, 61 (01) : 81 - 90
[8] USE OF DYNAMIC LIGHT-SCATTERING TO ASSESS CRYSTALLIZABILITY OF MACROMOLECULES AND MACROMOLECULAR ASSEMBLIES
FERREDAMARE, AR
BURLEY, SK
[J]. STRUCTURE, 1994, 2 (05) : 357 - 359
[9] FIKRIG E, 1992, J IMMUNOL, V148, P2256
[10] PROTECTION OF MICE AGAINST THE LYME-DISEASE AGENT BY IMMUNIZING WITH RECOMBINANT OSPA
FIKRIG, E
BARTHOLD, SW
KANTOR, FS
FLAVELL, RA
[J]. SCIENCE, 1990, 250 (4980) : 553 - 556

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