ENRICHMENT AND ANALYSIS OF DESMOSINE AND ISODESMOSINE IN BIOLOGICAL-FLUIDS

被引：20

作者：

CUMISKEY, WR ^{[1
]}

PAGANI, ED ^{[1
]}

BODE, DC ^{[1
]}

机构：

[1] STERLING WINTHROP PHARMACEUT RES DIV, DEPT PHARMACOL, COLLEGEVILLE, PA USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1995年 / 668卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00092-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the enrichment and analysis of the elastin crosslinks, desmosine and isodesmosine, in biological fluids and tissues. It is adapted from published methods, offering improved recovery, sensitivity, resolution, and speed of analysis. Samples were hydrolyzed in 6 M HCl, after which the desmosines were enriched by CF1 cellulose chromatography and analyzed by HPLC with a C-18 column. Isodesmosine and desmosine were quantitated based on absorbance at 275 nm, with a limit of detection of approximately 30 pmol and recovery of approximately 66% in urine. Their t(R) values on our HPLC system were approximately 9 and 12 min, respectively. This method was used to evaluate the daily and weekly variation in the concentrations of desmosine and isodesmosine in human urine. The results suggest that this method can be used to process large numbers of biological samples for analysis of desmosine and isodesmosine.

引用

页码：199 / 207

页数：9

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