MONOCLONAL-ANTIBODIES TO ESCHERICHIA-COLI BETA-GALACTOSIDASE AND THEIR USE FOR DETECTION AND PURIFICATION OF RECOMBINANT EXPRESSION PRODUCTS

被引：4

作者：

DRABER, P

SLAVICKOVA, A

SLADECEK, M

VIKLICKY, V

机构：

来源：

HYBRIDOMA | 1992年 / 11卷 / 03期

关键词：

D O I：

10.1089/hyb.1992.11.385

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A panel of seven mouse monoclonal antibodies (BG-01 - BG-07) was prepared against beta-galactosidase derived from E. coli. The antibodies are beta-galactosidase specific, show no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beat-galactosidase fusion proteins expressed in lambda expression vectors. One of the antibodies allows a simple, one-step isolation of the fusion proteins directly from the crude bacterial lysates using immunoaffinity chromatography.

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页码：385 / 390

页数：6

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