MONOCLONAL-ANTIBODIES TO ESCHERICHIA-COLI BETA-GALACTOSIDASE AND THEIR USE FOR DETECTION AND PURIFICATION OF RECOMBINANT EXPRESSION PRODUCTS

被引:4
作者
DRABER, P
SLAVICKOVA, A
SLADECEK, M
VIKLICKY, V
机构
来源
HYBRIDOMA | 1992年 / 11卷 / 03期
关键词
D O I
10.1089/hyb.1992.11.385
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A panel of seven mouse monoclonal antibodies (BG-01 - BG-07) was prepared against beta-galactosidase derived from E. coli. The antibodies are beta-galactosidase specific, show no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beat-galactosidase fusion proteins expressed in lambda expression vectors. One of the antibodies allows a simple, one-step isolation of the fusion proteins directly from the crude bacterial lysates using immunoaffinity chromatography.
引用
收藏
页码:385 / 390
页数:6
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