SYSTEMATIC-APPROACH TO TREATMENT OF ENANTIOMERIC SEPARATIONS IN CAPILLARY ELECTROPHORESIS AND LIQUID-CHROMATOGRAPHY .1. INITIAL EVALUATION USING PROPRANOLOL AND DANSYLATED AMINO-ACIDS

被引:40
作者
PENN, SG
LIU, GY
BERGSTROM, ET
GOODALL, DM
LORAN, JS
机构
[1] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND
[2] PFIZER LTD,CENT RES,SANDWICH CT13 9NJ,KENT,ENGLAND
关键词
D O I
10.1016/0021-9673(94)80062-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A systematic approach is outlined for treatment of enantiomeric separations in capillary electrophoresis (CE) and Liquid chromatography (LC) using chiral mobile phase additives. General equations and data analysis methods are presented to relate mobilities or capacity factors to equilibrium constants in binding equilibria, and to maximise mobility or retention time differences as a function of selector concentration. The use of cyclohexanol as a competitor is shown to be beneficial in optimising chiral separations of species which bind strongly to beta-cyclodextrins. This general treatment has been applied with the test systems 1: propranolol and beta-cyclodextrin and 2: dansylated amino acids and beta-cyclodextrin. Chiral separations and binding constants, determined using LC with beta-cyclodextrin as a mobile phase additive or a chiral stationary phase, are compared with results using the same selector in CE for system 2. Mobile phase equilibria defined by CE reveal more complex stationary phase binding equilibria in LC. Our studies make a link between LC and CE which may allow rational separation strategies to be transferred between the two fields.
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页码:147 / 155
页数:9
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