A MULTIENZYME SYSTEM FOR A ONE-POT SYNTHESIS OF SIALYL T-ANTIGEN

被引：51

作者：

KREN, V ^{[1
]}

THIEM, J ^{[1
]}

机构：

[1] ACAD SCI CZECH REPUBL,INST MICROBIOL,BIOTRANSFORMAT LAB,CR-14220 PRAGUE 4,CZECH REPUBLIC

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1995年 / 34卷 / 08期

关键词：

ENZYME CATALYSIS; BETA-GALACTOSIDASE; OLIGOSACCHARIDE SYNTHESIS; SIALYLTRANSFERASE; T-ANTIGEN;

D O I：

10.1002/anie.199508931

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

With a new multienzyme system with cofactor regeneration based on a sequential action of glycosidase and glycosyltransferase, a semipreparative scale synthesis of the cell epitope Neu5Acα(2 → 3)Galβ(1 → 3)GalNAc 1 was achieved. Although the participating enzymes have maximum activities at very different pH values, a satisfactory optimization of the synthesis was possible. (Figure Presented.) Copyright © 1995 by VCH Verlagsgesellschaft mbH, Germany

引用

页码：893 / 895

页数：3

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